The University of Arizona
MIC 285 Principles of Microbiology: Information, Syllabus, Honors Section Syllabus.
Problem sets

Dr. B. A. Fane
Office: 102A VetSci / Micro Building
Phone: 626-6634
Email: bfane@u.arizona.edu
Office hours: Wednesday 2:00-3:00 and see below.

Text: Microbiology. Prescott, Harley and Klein, Sixth edition.

Objectives: The objective of this course is to give an introduction to selective topics in Microbiology. Microbiology is an extremely diverse discipline. The course will concentrate on five microbiology sub-disciplines, microbial structure, metabolism and physiology, genetics, virology and immunology.

Exams: There will be three hour exams and a final exam, each worth 100 points. Exam questions will primarily be short answer and short essay questions. If the average of any given exam is below 75%, that exam will be curved with the average being set at 75%. In addition there will be four quizzes, each worth 5 points.

Grade calculation: A student can receive a total of 420 points in the course. Grades, however, will be calculated by dividing the points obtained by 400.

Grading: 90%-105% A
80%-90% B
70%-80% C
below 70% E

The Honors Section will also have two short take home quizzes, each worth 50 points. Hence, grades will be calculated on 500 points.

Suggestions for doing well in this course:

General: Attend classes and compile a thorough set of lecture and study notes. Do not fall behind.

Before lecture: Do a preliminary reading of the assigned chapters and familiarize yourself with definitions, concepts and terms.

During lecture: Don't be embarrassed to ask questions or ask me to repeat myself. Take notes.

After lecture: Review your notes, and with the use of your text, be sure that you understood the lecture. If anything seems unclear, come to see me.

Additional Resources: My office hours will be 2:00-3:00 Wednesday or by appointment. Because I am often, but not always, available outside of office hours, feel free to come see me at anytime. If time permits, I will be glad to assist you.

Completion of the assigned problem sets is not required. They are located on the Microvet server under courses, and on my home page, also on the server. The best way to approach the problem sets is as a self-evaluation tool. Study the material first, close your book and class notes and then attempt the problems. If you cannot do a problem, then you obviously need to study the material more. This is an opportunity for you to correct any misunderstandings regarding the material before the exam and to remain current in the course!

The answers to the problem sets will be given during discussion sections. Four times during the course, a short 5-point quiz will be given at the beginning of the discussion section. These quizzes will not be announced beforehand. The quiz will be taken directly from the problem set. Since grades are calculated using 400 points, not 420, no make-up quizzes will be given.

Policy on missed exams: Students representing the University in an official capacity, as defined by the Student Handbook, must notify me before the exam to schedule an alternative date. Students who miss an exam due to illness or other emergency, must contact me the day of the exam to schedule a make-up exam. A doctor's note may be required. All make-up exams will be given at the end of the course.

Academic Dishonesty will result in an E grade.
 
 

Date
Class topics and assignments
Discussion and honors section topics and assignments
1/12-1/19 Introduction, microscopy, review of biological molecules. 
Readings:  chapter 1, chapter 2, pp. 18-21; chapter 5 "Isolation of pure cultures pp.104-107, appendix 1.
Problem set 1.
Date
Discussion
Honors
1/12 Continuation of morning lecture. Introduction, phase contrast microscopy, electron microscopy, Readings: Chapter 2, pp.21-22, 30-32.
1/19 Problem set 1 Atomic structure and X-ray crystallography (online reading), and smart drugs (online reading) 


1/21 - 1/24 Prokaryotic cell structure, organelles and functions. 
Readings: Chapters 3.
Problem set 2.
1/26 - 1/31 Microbial growth: nutrition and cultivation. 
Readings: chapter 5; chapter 6 "Oxygen Concentration" pp. 125-127.
Problem set 3.
Readings chapter 6 pp.110-116; "Temperature" 122-124.
Problem set 4.
Date
Discussion
Honors
1/26 Problem set 2          
Applying to graduate and professional schools. Guest
speaker Dr. E. Marchello.


2/2- 2/4
Control of microorganisms. Readings: chapter 7. problem set 5.
Date
Discussion
Honors
2/2 Problem sets, 3, 4, 5. 
Microbial Control. Guest Speaker: Dr. S. Billington.            

2/7 -2/9
Microbial Metabolism I - Energetics and enzymes. Readings: chapter 8, pp.150-158.
Problem set 6.
Date
Discussion
Honors
2/9 
Problems sets 3, 4, 5.
Review for exam.  
No honors discussion section.                                       

2/11 Exam I:  Covers material through and including the control of microorganisms.
2/14- 2/18
Microbial Metabolism II - Catabolic processes: generating energy 
Readings: chapter 9 pp. 168 - 184; appendix II; figure A11.1, A11.4
Problem set 6.
Date
Discussion
Honors
2/16
Problem set 6. 
Allosteric enzymes and enzyme regulation
Readings: chapter 8 pp. 159-163.

2/21- 2/25
Microbial Metabolism III- Biosynthesis - anabolic processes. Readings: chapter 10 pp. 200-210; 214 (Figure 10.25); 216-217.
Problem set 7. 
Date
Discussion
Honors
2/23
Problem sets 6, 7. 
Parasitology: Guest speaker. Dr. C. Sterling.          

2/28 - 3/4
Microbial Metabolism VI - Nucleic acid and protein synthesis. Readings: chapter 11 pp. 229- 233, chapter 12 pp. 260-266.
Problem set 7.
Date
Discussion
Honors
3/2
Problem sets 6, 7.      
Environmental Toxicology: Guest speaker: Dr. O. Selmin

3/7
Exam Review

3/9
Exam II Covers all of Microbial Metabolism.
No Discussion or Honors Section! Enjoy your spring break!
3/11
No class, enjoy your spring break!

3/21-3/28
Microbial Genetics
Readings: chapters 11; pp. 222-224; 235 -239; 242-247. Chapter 12; pp. 254-256.
Date
Discussion
Honors
3/23
Problem set 8.                        
Luria and Delbruck: The slot machine experiment. T4 lysozyme frameshift experiments and the triplet (multiple of three) genetic code.

3/30 - 4/6
Viral Structure and genetic material. Readings: chapters 16. Problem set 9.
Viral life cycles. 
Readings: chapters 17, pp. 372-380; chapter 18, pp. 388 -399. Problem set 9. 
Date
Discussion
Honors
3/30
Problem sets 8 and 9.
Cancer causing viruses.                                                 
4/6
Problem sets 9
Virus assembly.

4/8 - 4/13
Pathogenesis and nonspecific host resistance. 
Readings: chapter 34; chapter 31. Problem set 10. 
Date
Discussion
Honors
4/13
Problem set 10         
Bacterial virulence factors. Guest Speaker: Helen Jost. 

4/15 - 4/27
Specific host resistance. Readings: chapter 32. 
Problem sets 10 and 11. 
Date
Discussion
Honors
4/20
Problem sets 10, 11.               
Allergic responses:  Guest speaker: Dr. J. Decker.
4/27
Problem set 11
Epidemics: determining the cause, Influenza v. West Nile virus. Guest speaker: Dr. J. Collins.

4/29
Exam III Covers Microbial Genetics, Virology and the Immune System. 

5/2 - 5/4
Review for final
Date
Discussion
Honors
5/4
Review for final
No Honors section.                                       

5/6
Comprehensive final exam. 11:00 AM - 1:00 PM.