This assignment will allow you to apply your knowledge of immunology to a timely
problem: development of a practical and safe vaccine for dengue (DEN gee) fever.
Dengue is caused by Dengue Virus, a Flavivirus transmitted to humans by mosquito
bites. The Centers for Disease Control and Prevention has a web site at which
you can read more about this pathogen: http://www.cdc.gov/ncidod/dvbid/dengue/index.htm.
A critique is a scientific review of journal articles containing a summary of the articles in your own words and your ideas/conclusions about the work described. (see Reading and Writing About Immunology). Your critique will address two research papers describing vaccines to Dengue Virus. The assignment is worth 100 points and is due Wednesday, July 7 by noon.
Background Information on Dengue, Dengue Hemorrhagic Fever, and Vaccines
CDC Dengue Fever Homepage http://www.cdc.gov/ncidod/dvbid/dengue/index.htm
Dengue/Dengue Hemorrhagic Fever: The Emergence of a Global Health Problem. Emerging Infectious Diseases 1: 55-57 (1995) available at http://www.cdc.gov/ncidod/eid/vol1no2/gubler.htm
Parham Chapter 12 pp. 328-338
Rothman, A. L. (2004) Dengue: Defining Protective versus Pathologic Immunity. J. Clin. Invest. 113 (7):946-951 available at Rothman HTML or http://www.jci.org/cgi/content/full/113/7/946?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=Dengue&searchid=1088699446842_2431&stored_search=&FIRSTINDEX=0&journalcode=jci
Dengue Vaccine Research Papers
Guirakhoo, F. et al. (2004). Safety and Efficacy of Chimeric Yellow Fever-Dengue Virus Tetravalent Vaccine Formulations in Nonhuman Primates. J. Virol. 78 (9): 4761-4775. available as Guirakhoo HTML or http://jvi.asm.org/cgi/content/full/78/9/4761#T9
Raviprakash, K. et al. (2003) Needle-free Biojector Injection of a Dengue Virus Type I DNA Vaccine with Human Immunostimulatory Sequences and the GM-CSF Gene Increases Immunogenicity and Protection from Virus Challenge in Aotus Monkeys Virology 315: 345-352. Available at Raviprakash HTML or http://80-www.sciencedirect.com.ezproxy.ahsl.arizona.edu/science?_ob=ArticleURL&_udi=B6WXR-49NRMHF-4&_user=56761&_handle=B-WA-A-A-AE-MsSAYZW-UUA-AUEAWUDCDA-AUYYDYYBDA-VEZCEVYZW-AE-U&_fmt=full&_coverDate=10%2F25%2F2003&_rdoc=9&_orig=browse&_srch=%23toc%237165%232003%23996849997%23466658!&_cdi=7165&view=c&_acct=C000005238&_version=1&_urlVersion=0&_userid=56761&md5=61e6cdf15a384df8d669b3dbcd6143fb
Arial, Helvetica, sans-serif">The journals are available through the links as PDF files, for which you will need Acrobat Reader. To access these articles as HTML files electronically, go to the Arizona Health Sciences Center website at http://www.ahsl.arizona.edu/ . Click on Journals at the top of the page and then navigate to the journal and article you want. Access is automatic from on campus; from off campus you may need to used your Cat Card # for access.
The critique should be around 4 pages single-spaced text. Write it as an essay, but include the following information (make each of these a subsection of the report with subtitles):
Contact Dr. J for any questions you have about the assignment or the papers. Send to jdecker@u.arizona.edu as an attachment by noon Wednesday July 7. Make sure your name is in the document as well as in the title.
Supplementary Materials: Vaccines