ToolBox

Tutorials    Abbreviations     Glossary
MIC419 Home     webMIC419 Home     VSC519 Home

Contents

  Reading and Writing about Immunology

• Abbreviations
• Glossary
• Reading and Writing About Immunology
• Related Immunology Sites

  Techniques for Inducing Immunity

• Immunization
• Monoclonal Antibody Production

  Techniques for Evaluating Immunity

Normal Human Immune System Parameters

Purifying Antibodies (and Soluble Antigens)

• Affinity Chromatography
• Antibody Absorption

Assaying Antibodies

• Agglutination
• Blotting: Western
• Complement-Mediated Lysis
• ELISA
• Equilibrium Dialysis
• Gel Electrophoresis: PAGE, IEF
• Hemagglutination Inhibition
• Immunohistochemistry
• Immunoprecipitation
• Isoelectric Focusing
• RIA

Designer Antibodies

• Abzymes
• Genetically Engineered Antibodies
• Monoclonal Antibodies

Purifying Cells

• Affinity Chromatography
• Density Centrifugation (Ficoll/Hypaque™)
• Flow Cytometry
• Negative Selection

Assaying Cells

• ADCC (Antibody-Dependent Cell-mediated Cytotoxicity)
• Antigen binding: Tetramer MHC-Peptide
• Apoptosis
• Blastogenesis ³H Thymidine Incorporation)
• CBC
• Chemotaxis
• Cytokine synthesis (ELISPOT)
• Cytotoxicity ⁵¹Chromium Release)
• Flow Cytometry, FACS
• Leukocyte Adhesion
• Limiting Dilution Assays
• Neutrophil Chemotaxis
• Neutrophil Granule Enzyme Assays
• Skin Testing (Hypersensitivites)

Designer Mice

• Adoptive Transfer
• Cell Lines (table of mouse/human with cell type, MHC alleles, markers, and functions)
• Inbred and Congenic Mice
• Knock-out Mice
• Radiation Chimeras
• Thymectomy and Bursectomy
• Transgenic Mice

Assaying Gene Function

• Blotting: Northern, Southern
• Libraries: cDNA, Genomic, Phage Display
• Nuclease Digestion
• PCR (DNA Amplification), RT-PCR

  Materials for Evaluating Immunity

• Reagents
• Research Animals
• Human Research

Top

Tutorials   MIC419 Home     webMIC419 Home     VSC519 Home